VIT reduction increases life span, lipid storage, autophagy and lysosomal lipolysis. (A) QPCR analysis of the expression of vit genes in d-1 animals fed control bacteria or bacteria expressing dsRNA against vit-1 from hatching (N = 3 per condition). (B) Life span analysis of animals fed control bacteria or bacteria expressing dsRNA against vit during adulthood. (C) Oil-Red-O staining of d-20 wild-type fed control bacteria or bacteria against vit during adulthood (insets are corresponding micrographs of each treated group). (D) Quantification of GFP::LGG-1 foci in the proximal intestine of d-1 wild-type animals fed control bacteria or bacteria expressing dsRNA against vit from hatching. *, P < 0.001, N = 28 worms per condition, ±SEM, t test. (E) QPCR analysis of the expression of lysosomal acid lipase (lipl-1 to lipl-8) genes in d-3 animals fed control bacteria or bacteria expressing dsRNA against vit from hatching (N = 3 per condition). ±SD; *, P<0.05; N = 3, t test. (F) Measurement of lysosomal acid lipase activity in wild-type animals and animals with reduced vitellogenesis. Fluorometric values normalized to the wild type. ±SD; *, P < 0.05; N = 3 per condition, t test. Life-span analysis of atg-18(gk378) mutants (G), lipl-3(tm4498) (H) and lipl-4(tm4417) mutants (I), daf-12(rh61rh411) mutants (J), nhr-49(nr2014) mutants (K) and glp-1(e2141);nhr-80(tm1011) mutants (raised at the permissive temperature) (L) fed control bacteria or bacteria expressing dsRNA against vit during adulthood. Details on life span analyses are found in Table S3.