PMC

Relative change of SHBG. Protein levels of each time point are normalized to those at baseline immediately before levothyroxine administration (mean ± SEM). Black line: ELISA measurements; dashed line: proteomics data.

Relative change to baseline of proteins involved in the coagulation cascade. Lines indicate the correlation between protein abundance and fT₄ level: r ≥0.8 (black) and r ≤-0.8 (dark gray).

Evaluation of LC-MS/MS results for FIX and FXIII subunit B by ELISA. Protein levels of each time point are normalized to those at baseline immediately before levothyroxine administration (mean ± SEM). Black line: ELISA measurements; dashed line: proteomics data.

Kinetics of thyroid hormones fT₄, fT₃ and TSH levels after oral administration of 0.25 mg of levothyroxine per day. Hormone levels of each time point are shown as means ± SD. Horizontal lines indicate the maximum value of the reference interval for the specific TH (minimum value for TSH, respectively).

Schematic view of the coagulation cascade. Colored boxes highlight proteins with r ≥0.9 (dark red); r ≥0.8 (light red); r ≤-0.9 (green). Gray boxes: proteins were covered by the analysis, but displayed no significant correlation (0.8 ≥ r ≥ −0.8). A2M = α₂-Macroglobulin; F2 = coagulation factor II; FG = fibrinogen; KLKB1a = plasma kallikrein; PLG = plasminogen; SERPINA1 = α₁-antitrypsin; SERPINA5 = protein C inhibitor; SERPINC = antithrombin III; SERPIND1 = heparin cofactor II; TFPI = tissue factor pathway inhibitor; THBD = thrombomodulin; TPA = tissue plasminogen activator; UPA = urokinase plasminogen activator; VWF = von Willebrand factor.