Collagen deposition and leukocyte infiltration in lungs of PiZ mice. A, histochemical staining with trichrome. PiZ x GFP-LC3 (n = 2) is in the right panels, and control, GFP-LC3 (n = 2), age 6 months, is in the left panels. % area stained was 9.83 ± 0.92 for PiZ-GFP-LC3, n = 61 versus 2.83 ± 0.41 for control, n = 21; p < 0.0001). B, hydroxyproline content. The mean ± S.E. is shown for each group (n = 5–8). *, difference from control, p < 0.005; **, difference from PiZ, p < 0.005. C, TEM imaging. Sections from 2 mice each at 50,000× magnification. The thickness was significantly increased in PiZ (n = 6) versus control (n = 5) at age 6–12 months analyzing 27 and 25 fields, respectively (p < 0.0001). The arrows point to basement membrane. The control in each is GFP-LC3. D, immunostaining of leukocytes in the lungs of PiZ mice using anti-CD45 (pink). Quantitative morphometry was done for PiZ (n = 4) and the control (n = 3) at age 6 months, analyzing 10 and 9 fields, respectively, for % area stained in sections shown as the mean ± S.E. p = 0.0006 at the right. The control was GFP-LC3.