Novel NBD1 binding compounds. Representative SPR sensorgrams showing concentration‐dependent changes in resonance (colored lines in main panel or the markers in the inset panel) and fit to a binary association model (inset) with best fit line (black dashed lines) for (a) the representative α‐site binder Cp‐α1, (b) the representative β‐site binder Cp‐β1, and (c) the BIA compound. The average Kd and standard deviation between experiments were 350 ± 115 μM (n = 24), 230 ± 185 μM (n = 9), and 645 ± 56 μM (n = 27) for Cp‐α1, Cp‐β1, and BIA, respectively. Complete saturation was not achieved because of the limits of compound solubility compared with affinity. Instead, R max, representing complete compound saturation, was defined as the maximum response from ATP controls run prior to compound screening.