PMC

Rv2887 is in the MarR family. (A) Alignment of Rv2887 to the consensus sequence for Pfam01047 (MarR) and four other MarR family proteins (selected as the five most diverse members in the conserved domain database, including the consensus sequence) (). The bottom row is the consistency, as estimated by PRALINE (). (B) Secondary structure prediction from Phyre2 for Rv2887 (). (C) Secondary structure prediction for the C terminus of mutant 3. The black boxes in panels A and B indicate V61, which is mutated to alanine in mutant 2.

The 3,183-bp deletion in mutant 1, which includes Rv2887. (A) Coverage analysis of sequencing results for M. tuberculosis H37Rv and mutant 1, showing a lack of reads in this region for the mutant but not the parent H37Rv strain. (B) Confirmation of the deletion mutation. Lane 0, Invitrogen 1-kb Plus DNA ladder. Numbers over the bars indicate the size in base pairs of the ladder. Lane 1, PCR of genomic DNA from the parent H37Rv strain with primers outside the region of the deletion. Lane 2, PCR of genomic DNA from mutant 1 with the same primers as for lane 1. Lane 3, PCR of genomic DNA from H37Rv with primers inside the deletion (in Rv2887). Lane 4, PCR of mutant 1 genomic DNA with the same primers as for lane 3.