CyTOF analysis of CD45+ cells from murine colon. Cells were isolated from colon of 6–9 weeks old WT female C57Bl/6 mice and stained with a panel of 26 cell surface markers. The results were gated for live, single, CD45+ cells. Bh-SNE analysis and clustering were performed by Accense (http://www.cellaccense.com/) and the results were processed by GIMP. Colors indicate high levels of the following markers: green – TCRβ, CD3e (T cells), Orange – B220 (B cells), light blue – Ly6G (granulocytes), pink –Ly6C (monocytes), purple – CD64, F4/80 (macrophages), blue – clustered by Accence, different DC populations, gray – non-identified or non-specific cells. Red populations in zoom-in black squares indicate high levels of the marker written. Representative of at least four separate, independent experiments.