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1.
Fig. 3

Fig. 3. From: Cytoskeletal alterations associated with donor age and culture interval for equine oocytes and potential zygotes that failed to cleave after ICSI.

Multipolar and bipolar spindles in equine failed zygotes, with four poles imaged in the multipolar spindle. Blue, chromatin; green, tubulin. Scale bar= 20 μm.

Elena Ruggeri, et al. Reprod Fertil Dev. ;27(6):944-956.
2.
Fig. 4

Fig. 4. From: Cytoskeletal alterations associated with donor age and culture interval for equine oocytes and potential zygotes that failed to cleave after ICSI.

Tubulin multiasters in failed equine oocytes that failed to cleave after intracytoplasmic sperm injection, with representative high and low numbers of tubulin multiaster foci. Blue, chromatin; green, tubulin. Scale bar= 20μm.

Elena Ruggeri, et al. Reprod Fertil Dev. ;27(6):944-956.
3.
Fig. 5

Fig. 5. From: Cytoskeletal alterations associated with donor age and culture interval for equine oocytes and potential zygotes that failed to cleave after ICSI.

Equine oocytes that failed to cleave after intracytoplasmic sperm injection, with (a) low and high numbers of actin vesicles and (b) small and large areas of actin vesicles. Green, tubulin; red, actin. Scale bar= 20 μm

Elena Ruggeri, et al. Reprod Fertil Dev. ;27(6):944-956.
4.
Fig. 2

Fig. 2. From: Cytoskeletal alterations associated with donor age and culture interval for equine oocytes and potential zygotes that failed to cleave after ICSI.

Sperm-injected oocytes had different numbers of spindles after failing to cleave. Two spindles represent the female and male sets of chromosomes; one spindle represents the female set of chromosomes; no spindles were present after degeneration and decondensation of the spindle structures. Blue, chromatin; green, tubulin; red, actin. Scale bar= 20 μm.

Elena Ruggeri, et al. Reprod Fertil Dev. ;27(6):944-956.
5.
Fig. 1

Fig. 1. From: Cytoskeletal alterations associated with donor age and culture interval for equine oocytes and potential zygotes that failed to cleave after ICSI.

Equine oocytes cultured in vitro for 28 h (Time 0; predicted time of MII) with no tubulin multiasters and for a further 48 h (Time 48) with diffuse foci of tubulin asters. Blue, chromatin; Green, tubulin; Red, kinetochores. Scale bar = 20 μm.

Elena Ruggeri, et al. Reprod Fertil Dev. ;27(6):944-956.

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