PMC

Length distribution of the RRM-containing human gene products. We studied the protein length distribution of the human gene products that were identified as RRM-containing.

Overall schema of the methodology and analysis. The figure highlights the analysis performed on RRM-containing Pfam families and the methodology adopted to perform genome-wide survey in Homo sapiens genome.

Domain architectures of the RRM-containing human gene products. We studied the human gene products for the presence of the co-existing domains. The schematic diagram displays the most frequent domain architectures drawn using the software DOG 1.0.

Sequence identity within RRM families’ members. We studied the sequence diversity between the members within a RRM family. (a) It shows the average sequence identities of different families and (b) The distribution of percentage sequence identity of the seven RRM families in Pfam.

Enrichment analysis for biological processes. The RRM-containing human gene products were studied for their functional clustering based enrichment analysis using DAVID 6.7. The processes, which were observed to be enriched were related to mRNA processing, splicing and its regulation.

Length distribution within RRM family members in Pfam database. We studied the protein length distribution of RRM families’ members. (a) Average amino acid length of a RRM family and (b) The distribution of amino acid lengths of the members of the seven RRM families in Pfam.

Modular nature of RRM. Within a single polypeptide sequence, RRM domain was observed to occur multiple times in 108 of the human gene products. This figure highlights the distribution of different RRM domains and their multiple occurrences. RRM_1 is present most frequently (88 of the gene products) and is repeated twice within a single protein sequence.

Conservation mapping on RRM structures. The multiple sequence alignments for each of these families were employed to study sequence conservation and the conserved residues were mapped to the RRM structures (in green). However for the families RRM_2, RRM_4 and family-RRM, there is no structural representation and we therefore, used structure of RRM_1 family for mapping. (a) RRM_1 (1L3K, chainA), (b) RRM_2, (c) RRM_3 (1OWX, chainA), (d) RRM_4, (e) RRM_5 (1A9N, chain d), (f) RRM_6 (1WG5, chain a) and (g) RRM.