PMC

Summary of results concerning functional analysis of the activity of the HSPA2 promoter in transgenic mice testes. The upper diagram shows the structure of the HSPA2 transcription unit. The potential sequences implicated in regulation of the HSPA2 gene expression in rodent tissues are indicated. Note that only the regulatory sequences that were subjected to functional studies are shown. T1 (−353) and T2 (−116) transcription start sites. The bottom diagram shows the promoter fragments that were fused to a reporter gene and analyzed in transgenic mice testes. Transgene expression (positive); No transgene expression (negative). Activities of constructs 1 and 4 were reported in Scieglinska et al. (), those of constructs 2 and 3 were described in Widlak et al. (), construct 5 was evaluated in Dix et al. (), and finally, construct 6 was assessed in Scieglińska et al. (). Numbers are coordinates of DNA fragment position in relation to A (+1) in the ATG translation start codon. DEL deletion, MUT point mutations, tk thymidine kinase promoter