A) MCF-7-TNR cells were cultured for 96 hrs with or without exposure to SB203580, a p38 inhibitor for 72 hrs (p38-SB-L, -M, and -H indicate a dose at 10 μM, 25 μM, 50 μM, respectively). The RNA levels of HOTAIR were measured using qRT-PCR. A fold change was obtained by normalizing to the house keeping gene 36B4 and setting the values from the DMSO control group to one. B) The culture condition was identical to part A. Cell proliferation was assessed by counting the cells at seeding and harvest. A fold increase in the number of cells was obtained by calculating the ratios of the number of cells at 96 hrs post-seeding over the initial number of cells seeded. C) MCF-7-TNR cells were exposed to DMSO or SB203580 (p38-SB-H indicates a dose at 50 μM) for 1 hr. Immunoblots were carried out to examine the proteins levels of total p38, p38 with Thr180/Tyr182 phosphorylated (P-p38), total HSP27, and HSP27 with Ser82 phosphorylated (P-HSP27). D) MCF-7-TNR cells were exposed to SB203580 for 72 hrs (p38-SB-L indicates a dose at 10 μM and p38-SB-H indicates a dose at 50 μM). The protein levels of the indicated genes were measured using immunoblots. E) Similar to part A except that MCF-7-TNR cells were exposed to the SRC inhibitor Bosutinib (BOSU-L indicates a dose at 1 μM and BOSU-H indicates a dose at 3 μM). The RNA levels of HOTAIR were assessed as in part A. F) The culture condition was identical to part E. Cell proliferation was assessed as described in part B. G) Similar to party C except that MCF-7-TNR cells were exposed to Bosutinib (BOSU-H, 3 μM) for 1 hr. When presented, means and standard deviations were obtained from three independent experiments. ** and *** indicate a P value < 0.01 and 0.001, respectively.