Myocardial triglyceride (TG) turnover and content, acyl intermediates, and related protein levels of enzymes for TG dynamics. (A) Oleate supported elevated turnover in normal (SHAM) hearts and attenuated the drop in turnover in hypertrophied (TAC) hearts versus palmitate (n=6 for each group). (B) Palmitate failed to maintain normal levels of TG in TAC hearts, while oleate supported normal TG similar SHAM hearts (n=6 for each group). (C) Oleate induced greater 13C enrichment of TG than palmitate in both SHAM and TAC (n=6 for each group). (D) TAC hearts supplied palmitate contained lower DAG, versus SHAM (n=3 for each group). (E) Palmitate elevated C16 ceramide in TAC hearts, versus SHAM Palmitate and TAC Oleate (n=5 for each group). (F–H) Western Blot analysis of rate-limiting enzymes of TG synthesis, diglyceride acyltransferase 1 (DGAT1), and hydrolysis, adipose triglyceride lipase (ATGL) from whole tissue lysate. Calsequestrin (CALSEQ) served as a loading control (n=3 for each group; P=0.14 versus all other groups). (I) Mean time constants characterizing the saturable exponential phase of 13C TG enrichment (n=6 for each group). (J) Western Blot analysis of CD36 from isolated total cardiac membranes. Na+/K+ ATPase served as a loading control (n=3 for each group). White bar, SHAM; black bar, TAC. Error bars indicate mean ± SEM. *P<0.05, versus SHAM Oleate; †P<0.05, versus TAC Oleate; ‡P<0.05, versus SHAM Palmitate; “n.s.”, not statistically significant.