PMC

Evaluation of neuroprotection of unsubstituted, phenolic, methoxy-substituted, and selected carbamyolated compounds at 10 μM against glutamate-induced oxidative stress on HT-22 cells (cf. the for details).

(A) Time-dependent pattern of inhibition of BChE by compound 8b (10–50 nM). (B) Stability constants of the inhibitor–ChE complex (K_C) and rate constants of carbamoyl–ChE formation (k₃) of 8a,b and physostigmine, respectively.

Reagents: (i) (Cl₃CO)₂CO, dry THF, 40–50 °C, 3 h. (ii) CH₃I, N,N-diisopropylethylamine, N,N-dimethylacetamide, 40 °C, 24 h. (iii) Toluene/reflux, 24 h. (iv) LiAlH₄, THF, 70 °C, 3 h. (v) Ethyl(methyl)carbamic chloride, NaH, THF, rt or isocyanate, Et₃N, CH₂Cl₂, rt. (vi) Pyrrolidin-2-one, MW 100–200 W, 130 °C, 1 h. (vii) Benzylbromide, K₂CO₃, acetone, 70 °C, 24 h. (viii) CH₃I, dioxane, 90 °C, 24 h. (ix) LiAlH₄, THF, 70 °C, 3 h. (x) H₂, Pd/C, ethanol, rt, 24 h. (xi) Isocyanate, CH₂Cl₂, Et₃N, rt, 1–3 h.