Mediator accumulates at core promoters in the absence of Kin28 kinase activity (see also ). (a) Mediator (Gal11, green) and RNAPII (Rpb3, red) occupancy in wild type (WT) and kin28 ATP analog-sensitive (kin28-as) cells, both treated with NAPP1 (+NAPP1), along a segment of chromosome VII. TFIIB (purple) from WT cells is shown as a placeholder for core promoters. Zooms into regions around the PMA1 and SCW1 genes are shown at the bottom. Vertical dashed line indicates PIC positions and arrows point toward accumulation of Mediator in core promoter regions. (b) Average Mediator (Gal11, green) and RNAPII (Rpb3, red) occupancy in wild type (WT, solid traces) and kin28-as (dotted traces) cells, both treated with NAPP1, around the TSS of all genes with an Rpb3 average ORF occupancy >1 in WT cells (n=266). TFIIB (purple) is shown as a placeholder for core promoters. (c) Scatter plot of Mediator (Gal11) occupancy versus RNAPII (Rpb3) occupancy in wild type (WT, top) and kin28-as (kin28-as, bottom) cells, both treated with NAPP1. Gal11 occupancy is defined as the maximum Gal11 enrichment observed in the first kilobase upstream from the TSS for each gene. Rpb3 occupancy is defined as the average occupancy across the entire ORF. (d) Mediator (Gal11, green) and RNAPII (Rpb3, red) occupancy in wild type (WT) and kin28-as cells, both treated with NAPP1, around the RPL10 gene. TFIIB (purple) is shown as a placeholder for core promoters. The arrow indicates the accumulation of Mediator in the promoter of RPL10 in kin28-as cells.