(a) CD4 T cells from the spleens of the WT or Menin KO mice with a DO11.10 TG background were transferred into syngenic BALB/c mice, and the mice were then immunized with OVA (100 μg) plus LPS (10 μg) (n=5). The transferred OVA-TCR (KJ-1)-positive cells were monitored using FACS on the indicated days. The percentages (upper) and numbers (lower) of KJ1-positive cells are indicated (right). (b) The WT (CD45.1) and Menin KO (CD45.2) TH cells were mixed 1:1 and transferred into TCR-βδ KO mice (n=5). Representative staining patterns gated on TCR-β-positive cells (upper) and the percentage (lower) of CD45.1 and CD45.2 cells 4 weeks after transfer are indicated. (c) Quantification of eosinophils (Eos), neutrophils (Neu), lymphocytes (Lym), macrophages (Mac) and total cells in the bronchioalveolar (BAL)-fluid from the WT and Menin KO mice (n=5 per group) that were not immunized (ctrl.) or immunized with OVA plus alum (imm.). (d) The microscopic findings of the lungs from mice as in c, fixed and stained with haematoxylin and eosin. (e) The effects of Menin deficiency on L. monocytogenes (LM) infection. The bacterial burden in the spleen was determined 3 days after the primary (n=6) and secondary (n=8) infections, respectively. The data represent the findings of three (a,b) or two (c,d and e) independent experiments. *P<0.05, **P<0.01 (Student’s t-test).