(A) Relative PGC-1α mRNA abundance in human dermal microvascular ECs stimulated with VEGF (10 ng/mL), S1P (100 nM), or S-nitrosoglutathione (GSNO, 100 μM).
(B and C) Isolated MHECs from PGC-1α-/- (KO) mice are hypermigratory in scratch assays (B) and Transwell migration assays (C).
(D) Capillary formation of KO (n = 31) and wild-type (WT) (n = 16) mouse aortic explants.
(E) mRNA expression of known Notch target genes in ECs isolated from KO mice, versus wild-type controls.
(F) GTP-Rac1 pull-down assay in WT and KO MHECs.
(G) VEGF (10 ng/ml)-induced Transwell migration of KO and WTMHECs, with or without DPI (diphenyleneiodonium chloride, 10 μM), L-NAME (L-NG-Nitroarginine Methyl Ester), or Rac inhibitor (NSC23766, 30 μM). *p< 0.05 versus control. **p < 0.01. The symbol # indicates p < 0.01 versus KO treated with vehicle. Data are means ±SEM. n = 3–18 for all groups. See also .