PMC

Outline of the method and examples of resulting data. (a) Outline of the method used for multiplexed mapping of large numbers of transposon insertion lines. (b) Amplified DNA from the transposon–genome junction for three representative inserts. Line identities could be correctly decoded from the pooled samples. (c) Illumina sequencing data from pooled samples for 5′ end-associated sequences for the same three lines shown in b. These three lines were correctly decoded and matched the genomic positions determined by splinkerette PCR mapping. The sequences associated with lines 0359 (inserted in ATPalpha) and 0754 (inserted in CG30419) were decoded without any error correction, and the sequence associated with line 0606 (inserted in encore) was decoded with a 1 to 0 correction. The error is denoted by a red digit.