PMC

Localised necrosis following an envenoming bite to the foot by the viperid Fer de Lance, (Bothrops asper).

Ptosis and rhabdomyolysis (note the black urine) following an envenoming bite by South American rattlesnake (Crotalus durissus) in Brazil.

A terminal bouton in advanced stages of degeneration. Note the lesions in the plasma membrane (arrows) and the damaged mitochondria (stars).

Incisions applied to the hand and lower leg respectively in two victims of envenoming bites by unidentified snakes in Chittagong, Bangladesh.

Severe neurotoxicity and rhabdomyolysis (note the black urine) following an envenoming bite by a greater black krait, Bungarus niger in Bangladesh. The patient did not recover.

A cluster of six individual end-plates (labelled with FITC-conjugated α-bungarotoxin) innervated by the clustered intramuscular branching of a single motor axon (labelled with TRITC-conjugated anti-neurofilament Ab.

Wrist drop and foot drop, respectively, many months after the apparently successful treatment of victims of neurotoxic snake bites in Chittagong, Bangladesh.

Victim of an envenoming bite by an unidentified snake on admission at a tertiary referral hospital in Chittagong, Bangladesh. Note multiple tight ligatures applied to the arm.

The relative rates of loss of desmin and myosin from muscles at various times after the inoculation of the venom of Notechis scutatus. (From Harris et al. 2003 []). Reproduced with permission from the Publisher.

(A) Young boy with severe neurotoxic signs following a bite by a cobra (species unknown) in Bangladesh; (B) Full recovery 24 h later following treatment with antivenom.

TEM of a longitudinal section of a rat soleus muscle fibre labelled with a gold-conjugated Ab against notexin, an sPLA₂ from the venom of the Australian tiger snake, Notechis scutatus, three hrs after exposure in vivo to the toxin. Arrows indicate individual silver-enhanced gold particles. (From Dixon and Harris 1996 []). Reproduced with permission from the Publisher.

(A) Longitudinal section of murine muscle labelled with ammodytoxin A, an sPLA₂ from the venom of the long-nosed viper, Vipera ammodytes, conjugated with Alexa⁵⁴⁶ (red) and counter-labelled with FITC-conjugated α-Bungarotoxin to label junctional ACh receptors (green) (B) a laser scan of red and green channels to demonstrate localisation of sPLA₂ to the neuromuscular junction. (From Logonder et al. 2008 []). Reproduced with permission from the Publisher.

The density of synaptic vesicles in terminal boutons of rat neuromuscular junctions. Vesicle density was unchanged in muscles incubated in vitro with either botulinum toxin C or conotoxin ω-MVIIC. Incubation with β-bungarotoxin, an SPLA₂ toxin from the venom of Bungarus multicinctus, caused a significant fall in vesicle density. The fall was largely or completely prevented in muscles pre-treated with either botulinum toxin C or conotoxin ω-MVIIC before exposure to β-bungarotoxin. (From Prasarnpun et al. 2004 []). Reproduced with permission from the Publisher.

Glycerophospholipid structure and the site of action of phospholipases. The phospholipid molecule consists of a glycerol-3-phosphate (blue) esterified at positions sn-1 and sn-2 to non-polar fatty acids. Its phosphoryl group is esterified to a polar head group (x). Phospholipases A₁ cleave the ester bond at the sn-1 position. Phospholipases A₂ cleave the ester bond at the sn-2 position. Phospholipases B cleave the ester bonds at both sn-1 and sn-2 positions. Phospholipases C cleave the glycerophosphate bond and phospholipases D remove the polar head group. From The AOCS Lipid Library (http://lipidlibrary.aocs.org/animbio/phospholipases/index.htm).

Snakes frequently involved in major neuro-myotoxic envenoming in humans. (A) the Australian Tiger snake, Notechis scutatus and (B) the South American rattlesnake, Crotalus durissus terrificus, both cause neurotoxicity and rhabdomyolysis; (C) the Taiwanese Multi-banded krait, Bungarus multicinctus causes severe neurotoxicity but no myotoxicity; (D) the Beaked sea snake, Enhydrina schistosa, causes severe myotoxicity but rarely neurotoxicity in human subjects.

(A). A group 1A phospholipase A₂ with phospholipid substrate modeled in the active site. The active site residues His-48 and Asp-99 and the bound Ca²⁺ is shown in purple. Ca²⁺ is bound by Asp-49 as well as the carbonyl oxygens of Tyr-28, Gly-30 and Gly-32. Aromatic residues are shown in white; (B). Model of the lipid binding of the group 1A PLA₂ is shown with residues on the interfacial binding surface Tyr-3, Trp-19, Trp-61 and Phe-64 shown in stick form. From Burke and Dennis 2008 [].

Longitudinal sections of rat soleus muscles 24 h after the inoculation, in vivo, of the venom of the Greater black krait, Bungarus niger. Sections were labelled with TRITC-conjugated α-Bungarotoxin to label junctional ACh receptors (red) and FITC conjugated Ab to neurofilament protein to label motor axons (green). (A) control image; (B–E) Progressive breakdown of the terminal innervation at the neuromuscular junction. Note the preservation of the junctional ACh receptors (From Faiz et al. 2010 []). Reproduced with permission from Publisher.

TEM images of terminal boutons on murine muscle fibres previously exposed to a gold-labelled sPLA₂ from the venom of the horned viper (Vipera ammodytes ammodytes). The control bouton (A) is not decorated; Bouton B shows particles within the synaptic cleft and folds. Bouton C shows particles in the synaptic cleft and the bouton itself. Enlarged images (D–I) show particles associated with vesicle–like structures within the bouton or with mitochondria (open arrows). The association between label and vesicle-like structures suggest that uptake might occur during the recycling of synaptic vesicles and endocytosis. (Modified from Logonder et al. 2009 []). Reproduced with permission from the Publisher.

(A–C) Transverse sections of soleus muscles stained with haematoxylin and eosin (H&E). (A) control; (B,C) Three and 24 h respectively after exposure, in vivo, to notexin, an sPLA₂ from the venom of the Australian tiger snake, Notechis scutatus. Note the early inflammatory response and the later degeneration of the muscle fibres; (D) Longitudinal section at 24 h stained with procion yellow. This dye is excluded from cells with an intact plasma membrane. Note that it has entered the muscle fibres and stained the congealed, hyper-contracted myofilaments.

(A–C) Transverse sections of soleus muscles stained with H&E. A. control. B, C. Four and 28 days respectively after exposure, in vivo, to notexin, an sPLA₂ from the venom of the Australian tiger snake, Notechis scutatus. Note the rapid growth of the muscle fibres and the continuing presence of centrally located myonuclei; (D) As above 28 days after exposure in vivo to the venom of the Fer de Lance, Bothrops asper, a viperid snake that causes extensive soft tissue necrosis (see ). Note the immature appearance of the regenerating muscle fibres and the extensive infiltration of connective tissue.