(A-C) Uptake (24h) of PKH26-labeled T-MV from MCF-7 into MΦ: (A) fluorescence/bright field (10×, inserts = controls), (B) confocal microscopy (40×, green = calcein). (C) Quantification of PKH fluorescence in MΦ and sn by flow cytometry (means ± SD, n=5, *p<0,01, **p<0,05). (D) Preincubation (2h) with dynasore (12,5μM) inhibits PKH26-labeled T-MV uptake (flow cytometry, n=3, mean±SD, *p<0,05), but does not counteract T-MV-induced WNT5A upregulation in MΦ (E) (qRT-PCR, means ± SD, n=9). (F) Uptake of PKH26-labeled (red) MΦ-MV into MCF-7 cells after 24h (fluorescence/bright field microscopy, 10x, inserts = controls). (G) Western blot: Wnt 5a delivered by MV from Wnt 5a-L cells as well as recombinant human Wnt 5a (100 ng/ml) are detectable in whole MCF-7 lysates. (H) No induction of WNT5A mRNA in MCF-7 by rhWnt 5 and MV from wt as well as Wnt 5a-L cells (qRT-PCR, means ± SD, n=9).