PMC

VSMC from SHR and WKY rats were incubated in the absence (control) or presence of various concentrations of C-ANP_4–23 (A), NSC 625987 (B) or NU2058 (C) for 16 h. Thymidine incorporation was determined as described in “”. Results are expressed as % of WKY CTL, taken as 100%. Values are means ± SE of 5 separate experiments. *** P<0.01, ***P<0.001 vs WKY CTL, #P<0.05, ##P<0.01 vs SHR CTL.

VSMC from SHR and WKY rats were incubated in the absence (control) or presence 10^-7M of C-ANP_4–23 for 16 h. The cell lysates were prepared and used for Western blotting using specific antibodies against Giα-2 (A) and Giα-3 (B) as described in “”. Results are expressed as % of WKY CTL, taken as 100%. Values are means ± SE of 5 separate experiments. **P<0.01, ***P<0.001.

VSMC from SHR and WKY rats were incubated for 16 h in the absence (control) or presence of pertussis toxin (PT, 10 ng/ml). The cell lysates were used for Western blotting as described in “”. Results are expressed as % of WKY CTL, taken as 100%. Values are means ± SE of 5 separate experiments. *P<0.05, **P<0.01 ***P<0.001.

VSMC from SHR and WKY rats were incubated in the absence (control) or presence of different concentrations of C-ANP_4–23 for 16 h. The cell lysates were prepared and used for Western blotting using specific antibodies against pERK1/2 (A) and pAKT (B) as described in “”. Results are expressed as % of WKY CTL taken as 100%. Values are means ± SE of 5 separate experiments. *P<0.05, **P<0.01.

VSMC from SHR and WKY rats were incubated in the absence (control) or presence 10^-7M of C-ANP_4–23 for 16 h. The cell lysates were prepared and used for Western blotting using specific antibodies against pRb (A) and Rb (B) as described in “”. Results are expressed as % of WKY CTL taken as 100%. Values are means ± SE of 5 separate experiments. **P<0.01.

VSMC from SHR and WKY rats were incubated for 16 h in the absence (control) or presence of 1 µM wortmannin WM). The cell lysates were prepared and used for Western blotting using specific antibodies against cyclin D1 (A), cdk4 (B), cdk2 (C) and pRB (D) as described in “”. Results are expressed as % of WKY CTL taken as 100%. Values are means ± SE of 5 separate experiments. *P<0.05, **P<0.01, * **P<0.001.

VSMC from SHR and WKY rats were incubated in the absence (control) or presence of C-ANP_4–23 (10⁻⁷M) for 16 h. The cell lysates were prepared and used for Western blotting using specific antibodies against cyclin D1 (A), cdk4 (B), cyclin A (C), cyclin E (D) and cdk2 (E) as described in “”. Results are expressed as % of WKY CTL taken as 100%. Values are means ± SE of 5 separate experiments. *P<0.05, **P<0.01, ***P<0.001.

One week old SHR and age matched WKY rats (control) were injected intraperitoneally with C-ANP_4–23 (10 nmol/Kg of body weight) twice weekly up to 8 weeks as described in “”. After eight weeks of treatment, the rats were sacrificed and aortic VSMC from SHR and age-matched WKY (control groups) and C-ANP-_4–23-treated groups were cultured and thymidine incorporation was determined as described in “”. Results are expressed as % of WKY CTL, taken as 100%. Values are means ± SE of 3 separate experiments.. *** P<0.001 vs WKY, ^### P<0.001 vs SHR.

VSMC from SHR and WKY rats were incubated for 16 h in the absence (control) or presence of 10 µM PD 98059 (PD). The cell lysates were prepared and used for Western blotting using specific antibodies against cyclin D1 (A), cdk4 (B), cdk2 (C) and pRB (D) as described in “”. Results are expressed as % of WKY CTL taken as 100%. Values are means ± SE of 5 separate experiments. *P<0.05, **P<0.01, ***P<0.001.