PMC

A) Normal eye staining. ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer. B–E) Staining for injured (Inj) eyes at 3-day, 1-week, 2-week, and 4-week post injury, respectively. F–I) Staining for hUCBSC transplanted (Inj+SC) eyes at 3-day, 1-week, 2-week and 4-week post surgery, respectively. J) Comparison of RGC number. ^* p<0.05, ^** p<0.001 vs. injury group. N = 5. A scale bar measures 100 microns.

A) Representative RT-PCR of CHOP expression. CHOP mRNA expression was detected 3 hrs, 12 hrs, 24 hrs, 48 hrs and 7 days post surgery in the injury (Inj) and hUCBSC transplantation (Inj+SC) groups. B) Comparison of the band-pixel values of CHOP mRNA in injury and hUCBSC transplantation rats. ^* p<0.001 vs. hUCBSC transplantation. N = 5.

A) Representative RT-PCR of GRP78 expression. GRP78 mRNA expression was detected 3 hrs, 12 hrs, 24 hrs, 48 hrs, 72 hrs and 7 days post surgery in the injury (Inj) and hUCBSC transplantation (Inj+SC) groups. B) Comparison of the band-pixel values of GRP78 mRNA in injury and hUCBSC transplantation rats. ^* p<0.001 vs. injury group. N = 5.

A) F-VEP in normal eye. B) Representative F-VEP in eye injured for 1 hr, 21 days (C), and 28 days (D). E) Representative F-VEP in the hUCBSC-transplanted eyes for 1 hr, 21 days (F), and 28 days (G). H) The amplitude of F-VEP at different time points in injury and hUCBSC transplantation groups. I) Latency of F-VEP at different time points in injury and hUCBSC transplantation groups. N = 5.

A) Representative TUNEL staining of normal retina, injured retina 7 days after injury, and hUCBSC-transplanted retinas 7 days after surgery. TUNEL-positive cells were seen in inner nuclear layer and ganglion cell layer. Arrows indicated the TUNEL-positive cells (Brown). ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer. B) TUNEL-positive cells counting in retina in RGC layer at different time points in injury and hUCBSC transplanted rats. N = 5. ^* p<0.05, ^** p<0.01 vs. 3 hrs. ^# p<0.05, ^## p<0.01 vs. injury group.