PMC

A: Data analyzed using GEO accession: GSE23558 demonstrate genes associated with structural molecular activity in GBCs. B: qRT-PCR analysis showed more than 15 fold downregulation of KRT76 expression in tumors compared to normal oral tissue.

IHC staining for K76 expression in hamster oral epithelium of A: Control group B: Hyperplastic lesion, C: Tumor with respective isotype controls. Magnification 100X (Scale: 100 µm).

Representative Immunofluorescent staining of A: Normal oral tissue, B: OPL, C: Well differentiated tumor, D: Poorly differentiated tumor. K76 (Stained green, Alexa fluor 488), Nuclei stained with DAPI (pseudo red). Magnification 200X (Scale: 50 µm).

Representative IHC staining on A: Normal buccal mucosa, B: Oral Premalignant Lesions and OSCC (C: well differentiated, D: Moderately differentiated, E: poorly differentiated), with respective isotype control. Magnification 100X (Scale: 100 µm).

A: Significant downregulation of K76 was observed in OSCC and OPL compared to normal. B: Kaplan–Meier plot for DSS of gingivobuccal cancer patients with respect to K76 IHC staining intensity. Footnote: *All normals showed more than +2 grade stain.

K76 antibody specificity was determined by IHC on Oral epithelium of KRT76-KO mice which did not show any staining (A), whereas wild type mice of same strain showed moderate staining (B), with respective isotype control (left panel A & B). Histological observation of H & E stained buccal epithelium demonstrated hyperplastic changes and increased keratinization in KO (C), compared to WT (D). Magnification 100X (Scale: 100 µm); selected area under 200×magnification.