PMC

High-dose rapamycin does not affect seizure frequency. A Histograms of seizures versus days of recording in a group of mice (1–10) that were video-monitored together in a terrarium with slots separating individuals. Recordings began 1 month after status epilepticus. Only seizures of grade 3 or greater () were counted. Plotted values indicate number of seizures observed per 9 h recording period. B Average seizure frequency. There was no significant difference (p = 0.845, t test). Error bars indicate s.e.m.

High-dose rapamycin does not prevent hilar neuron loss. Nissl-stained sections of the dentate gyrus of a naïve control mouse (A), a mouse that experienced pilocarpine-induced status epilepticus and was treated with vehicle (B), and a mouse that experienced status epilepticus and was treated with 10 mg/kg rapamycin (C). m = molecular layer, g = granule cell layer, h = hilus, CA3 = CA3 pyramidal cell layer. D Average number of hilar neurons per hippocampus. *p < 0.05 compared to other groups (ANOVA on ranks with Dunn’s method). Vehicle and rapamycin groups are not significantly different. Error bars indicate s.e.m.

Dose-dependent effect of rapamycin on mossy fiber sprouting but not seizure frequency in pilocarpine-treated mice. Data from and the present study. A Percent mossy fiber sprouting was calculated by subtracting the average percentage of the molecular layer plus granule cell layer that was Timm-positive in naïve control mice and normalizing by the average value of mice that had experienced status epilepticus and were treated with vehicle for 2 months. Averages of all groups are significantly different from others (p < 0.05, ANOVA with Student-Newman-Keuls method). Error bars indicate s.e.m. B Percent seizure frequency was calculated by normalizing by the average of the vehicle-treated group.

High-dose rapamycin blocks mossy fiber sprouting and suppresses dentate gyrus hypertrophy. Timm-stained sections of the dentate gyrus of a naïve control mouse (A), a mouse that experienced pilocarpine-induced status epilepticus and was treated with vehicle (B), and a mouse that experienced status epilepticus and was treated with 10 mg/kg rapamycin (C). m = molecular layer, g = granule cell layer, h = hilus, CA3 = CA3 pyramidal cell layer. Asterisks in A1–C1 indicate areas shown at higher magnification in A2–C2. D Mossy fiber sprouting along the septotemporal axis of the hippocampus. Values indicate mean ± s.e.m. E Average levels of mossy fiber sprouting. *p < 0.05 compared to other groups (ANOVA on ranks with Dunn’s method). Control and rapamycin groups are not significantly different. F Average volumes of the dentate gyrus. *p < 0.05 compared to other groups (ANOVA on ranks with Dunn’s method). Control and rapamycin groups are not significantly different.