PMC

Overall structure of the complex. DPP4 extracellular domain consists of N-terminal eight-bladed β-propeller domain (green) and C-terminal α/β-hydrolase domain (orange). MERS-CoV RBD contains a core (cyan) and a receptor-binding subdomain (purple). The disulfide bonds are drawn as yellow sticks and the N-linked glycans are drawn as pink sticks.

Structural comparison between MERS-CoV RBD and SARS-CoV RBD. Domain structures of MERS-CoV S1 (A) and of SARS-CoV S1 (B). (C) Structure of MERS-CoV RBD. The receptor-binding subdomain is colored in purple and the core subdomain is colored in cyan. (D) Structure of SARS-CoV RBD (PDB code 2AJF). The receptor-binding subdomain is colored in purple and the core subdomain is colored in wheat. (E) Schematic illustration of MERS-CoV RBD topology. β strands are drawn as arrows and α helices are drawn as cylinders. The disulfide bonds are drawn as yellow sticks. (F) Schematic illustration of SARS-CoV RBD topology. β strands are drawn as arrows and α helices are drawn as cylinders. The disulfide bonds are drawn as yellow sticks.

Binding interface. (A) DPP4 contacts the MERS-CoV RBD with its blades 4 and 5 in the N-terminal eight-bladed β-propeller domain. Patch 1 is centered around the C-terminal end of the long linker connecting β6 and β7 strands in MERS-CoV RBD. Patch 2 has a gently concaved outer surface in MERS-CoV RBD that contacts a linker containing a short α helix between blades 4 and 5 of DPP4. Amino acid interactions in patch 1 (B), and in patch 2 (C).

Effect of residue substitution on MERS-CoV RBD binding to DPP4 (A) and entry efficiency of pseudotyped viruses (B). (A) SDS-PAGE analysis of co-purified complexes of wild-type or mutant forms of His-tagged RBD and untagged DPP4. The actual residue changes in the RBD are indicated above each lane. The DPP4 untagged serves as a negative control to exclude nonspecific binding of untagged DPP4 with Ni-NTA resin. (B) Entry efficiency of pseudotyped viruses bearing the wild-type and mutant forms of viral spike glycoprotein. The percentage of entry efficiency was calculated on the basis of luciferase activity of mutant viruses versus that of the wild-type virus. Soluble RBD (∼150 μg/ml) and DPP4 (∼150 μg/ml) were also tested for their inhibitory activity against wild-type virus. One irrelevant soluble protein with the same concentration was used as a negative control. Error bars represent SD of two replicate experiments.