PMC

Kaplan-Meier curves for cancer-specific survival after radical cystectomy according to UBE2C status in bladder urothelial carcinoma. A: All cases (N=82). B: Cases with muscle-invasive bladder urothelial carcinoma (N=57).

UBE2C expression in non-neoplastic bladder mucosa and bladder urothelial carcinoma (UC). A: Normal bladder mucosa is negative for UBE2C. Arrow indicates UBE2C-positive inflammatory cell. B: High-grade invasive UC with weak nuclear UBE2C staining. Weak staining was categorized as negative in this study. C: UBE2C-positive high-grade invasive UC. D: UBE2C-positive lymph node metastasis of UC. A-D: Magnification, ×400.

Expression of UBE2C protein and the effect of UBE2C suppression in UM-UC-3 bladder cancer cell line. A: UM-UC-3 cells were transfected with control siRNA or two sequences of UBE2C siRNA and cultured for 48 h. Cells were lysed and UBE2C protein levels were detected by western blotting. β-actin expression was used to control equal protein loading. B: At 72 h after siRNA transfection, the viability of UM-UC-3 cells was determined, as described in Materials and Methods. Columns, absorbance; bars, standard deviation. *: p<0.01 compared with control siRNA.