[A–I] The transcript levels from qRT-PCR and digital gene expression analysis of differentially expressed genes: MCM3 [A], MCM5 [B], POLD2 [C], WOX4 [D], VND7 [E], MYB85 [F], CAD [G], CCR [H], and PETE1 [I]. Relative expression levels were calculated by qRT-PCR with actin as a standard. Columns and bars represented the means and standard error of relative expression levels (n = 3) respectively (Y axis at left). Lines represented the means of RPKM value calculated by digital gene expression analysis (Y axis at right). The x-axis indicated the phases of vein development. RPKM, Reads Per kb per Million reads.