Shown are the Pearson correlation coefficient between each library (columns) and the control Total library for either all transcripts (top row) or for transcripts with (a) different lengths; or (b) different GC content. The number of transcripts expressed in the control Total library in bins with length < 1,000, 1,000–5,000, and > 5,000 was 3,716, 38,088, and 7,050, respectively. The number of transcripts expressed in the control Total library in bins with GC content < 37%, 37–62%, and > 62% was 2,358, 42,660, and 3,836, respectively.