PMC

For each condition, three biological replicate samples were tested in triplicate qPCRs.

Slow-killing conditions were used for each strain and death of C. elegans was measured every 24 h for a total of 72 h. Exposure to bacterial lawn represents when L4 or young adult hermaphrodite C. elegans were added to pathogenic plates. Values represent the results from at least three biological replicates. Error bars represent standard deviation, and lack of error bars means a standard deviation of zero.

A. Air-liquid interface assay of biofilm formation of P. aeruginosa PA14 wild type and its deletion mutants, ΔPA0765-0757, ΔPA2070 and ΔPA5033. The images of biofilms at 200 X magnification were taken by phase-contrast microscopy after 24 h of growth at 37°C in M63-arginine medium. B. Quantification of biofilm formation by measurement of the biofilm-associated crystal violet (OD₅₅₀). The data shown are the mean ± standard deviation from two separate experiments (each with quadruplicate samples) using the microtitre plate biofilm assay and display no significant difference (p>0.05) among the four groups as determined by one-way ANOVA.