SW620 cells were treated with γ-tocotrienol, FH535 or PTX for 6 and 24 h. The morphological changes of SW620 cells were detected under a light microcopy (I, Magnification: ×100), a fluorescent microscope stained with DAPI (II, magnification: ×200) and AO/EB (III, magnification: ×100). The cells treated with control (A), 15 (B), 30 (C), 45 (D) and 60 µmol/L (E) for 24 h as well as 60 µmol/L of γ-tocotrienol (F) for 6 h, or the cells treated with PTX at doses of 0(A), 0.2(B), 0.4(C), 0.8(D), 1.2(E) µmol/L, or FH535 at doses of 0(A), 0.625(B), 1.25(C), 2.5(D) µmol/L and DMSO (E) for 24 h. After treatment, the cells showed the slow growth, loosed tight contacts amongst cells and reduction of cell size, turn round of cell shape and cytoplasmic vacuolar when compare a spindly and large and grew close together, with prominent nucleoli and abundant cytoplasm in control group. Red arrows indicate the vacuoles.