(A) Diagram of rapamycin treatment for morphology examination. (B) Compared to control neurons, the adult-born neurons with Disc1 knockdown have increased number of primary dendrites which is prevented by rapamycin treatment (***P<0.001, Two-way ANOVA). Numbers associated with bar graph indicate the number of neurons examined from at least 3 mice from each group. (C) Positioning of adult-born neurons in DG. Layer 1, layer 2 and layer 3 refer to the inner, the middle, and the outer layers of granule cells in DG, respectively; layer 4 refers to the molecular layer. (D) Sample traces recorded from GFP+ neurons in response to 800 ms 100 pA current injection before and after the addition of TTX (1 μM) at 14 days after virus injection. Scale bar: 50 mV and 200 ms. (E) Quantification of the total number of action potentials with 800ms stimulation (Con/veh, Cont/rap and DISC1/veh: n=5, DISC1/rap n=8; **P<0.01, Two-way ANOVA). (F) The percentage of exploration time during the object-place recognition test at 24h after training. Cont/veh and DISC1/rap mice spent significantly more time exploring the object at the new location (Con/veh n=14, Cont/rap n=12, DISC1/veh n=14, DISC1/rap n=15; ***P<0.001, one sample t-test compared to 50%). (G) In the forced swim test, the immobile time of DISC1/veh mice (n=11) was significantly higher than Cont/veh mice (n=12), and this increased immobility was prevented by rapamycin treatment (DISC1/rap n=9). Two-way ANOVA, *P<0.05, ***P<0.001. (H) DISC1/veh mice spent less percentage time in the open arms of the plus maze than Cont/veh mice (Cont/veh n=16, DISC1/veh n=17; *P<0.05). There was no significant difference between DISC1/rap and Cont/rap (DISC1/rap n=15, Cont/rap n=13, P>0.05, Two-way ANOVA). All data are shown as means ± s.e.m.