A) Dried or freshly-coated plates (white and gray symbols, respectively) were tested at different time points by assaying a set of 3 weak-positive and 3 negative samples (average = circles and squares, respectively). B–D) Fresh solutions of the calibrator were tested in triplicate at different time points, using fresh (grey circles) or stored dilutions of the kit reagents kept at room temperature (white) or 37°C (black symbols). All values were normalized with regard to the value obtained with the fresh reagent. B) Stability of the calibrator: triangles, PBS, 5% glycerol, 0.1% Kathon. C) MAb Eg9 stability: squares, PBS-T; triangles, PBS-T, 0.1% Kathon. D) Stability of the peroxidase anti-mouse IgG antibody: squares, PBS, 0.1% BSA; triangles, PBS, 0.1% BSA, 0.1% Kathon, 0.1 mM 3,3′,5,5′-tetramethylbenzidine (TMB).