PMC

Quantitative RT-PCR analysis of AGL62, PHE1, AGL90, AGL36, AGL40 and AGL28. Error bars indicate s.e.m.

Quantitative RT-PCR analysis of MEA and FIS2 expression in seeds derived from osd1×2n crosses at 1–4 days after pollination (DAP). Error bars indicate s.e.m.

Sections of seeds from 2n×2n (left panels), mea/MEA×2n (middle panels) and mea/MEA; osd1/osd1×2n (right panels) crosses at 6–12 days after pollination (DAP). Bar = 100 µm. Images of mature seeds are shown in bottom panels. Bar = 0.5 mm.

(A) Distribution of seed sizes from different crosses. A minimum of 250 seeds was analyzed for each cross. Inset shows percentage of seeds larger than 0.14 mm². (B) Percentages of normal, enlarged and collapsed seeds from different crosses. Numbers in parenthesis correspond to numbers of analyzed seeds.

(A) Quantitative RT-PCR analysis of PHE1, AGL62, AGL90, AGL40, AGL28 and AGL36 in seeds derived from crosses 2n×2n, nrpd1a/nrpd1a×2n, osd1/osd1×2n, and nrpd1a/nrpd1a; osd1/osd1×2n at 3 DAP. Error bars indicate s.e.m. (B) Distribution of seed sizes from different crosses. A minimum of 250 seeds was analyzed for each cross.

Quantitative RT-PCR analysis of AGL62, PHE1, AGL90, AGL40, AGL28 and AGL36 at 8 DAP in mea and fis2 seeds (upper and lower panel, respectively). Enlarged seeds with a fis phenotype were selected as 3n mea and fis2 seeds from mea/MEA; osd1/osd1×2n and fis2/FIS2; osd1/osd1×2n crosses, respectively. Diploid mea seeds are a mixture of wild-type and mea seeds. Error bars indicate s.e.m.

Venn diagrams showing overlap of genes being deregulated in seeds derived from osd1×2n crosses with genes deregulated in seeds of 4n×2n crosses (upper panel); overlap of deregulated genes in seeds derived from osd1×2n crosses with H3K27me3 target genes in the endosperm (middle panel), and genes that are upregulated in fis2 mutant seeds (lower panel). In the upper panel only deregulated genes that are present on the ATH1 array have been used to calculate the overlap.

(A) Size of seeds derived from crosses agl62/AGL62; osd1/osd1×wild type and diploid seeds from crosses agl62/AGL62×wild type. Average seed size of different genotypes is shown. Wild-type (+) and mutant (−) allele frequencies of AGL62 in the endosperm are shown below the x axis. Numbers on top of bars refer to numbers of analyzed seeds. Error bars indicate s.e.m. (B) Images of triploid seeds from the three categories shown in (A). Bar = 0.1 mm.

(A) Seeds from crosses of Col 2n×2n (upper panel) and osd1×2n (diploid and triploid seeds, middle and lower panel, respectively. Ploidy refers to ploidy of the embryo). Bar = 0.5 mm. (B) Average seed size (upper panel) and seed weight (lower panel) of different crosses. Numbers on top of bars correspond to number of analyzed seeds. For seed weight the average of 100 seeds was calculated in triplicates. Error bars indicate SD. The reference line corresponds to wild-type seed size and weight. (C) Number of endosperm nuclei in Col 2n×2n and osd1×2n crosses. n = 5. Error bars indicate SD. (D) Sections of seeds from Col 2n×2n and osd1×2n crosses. Bar = 100 µm.