PMC

Solithromycin inhibition of ribosomal subunit formation in S. pneumoniae (a), MSSA (b), MRSA (c), and H. influenzae (d). Percentages of the total gradient radioactivity in the 30S and 50S subunits are shown. The horizontal line indicates the 50% value. Arrowheads indicate IC₅₀s. Standard errors are indicated (n = 2).

Inhibition of protein synthesis rate, growth rate, and CFU by solithromycin for S. pneumoniae (a), methicillin-susceptible S. aureus (MSSA) (b), methicillin-resistant S. aureus (MRSA) (c), and H. influenzae (d). Percentages of the control growth rate, cell number, and protein synthesis rate are shown. Arrowheads indicate IC₅₀s. Standard errors are indicated (n = 2).

Agilent gel electropherogram of total RNA samples from control and solithromycin-treated cells. Lane 1, S. pneumoniae, no antibiotic; lane 2, S. pneumoniae plus solithromycin; lane 3, MSSA, no antibiotic; lane 4, MSSA plus solithromycin; lane 5, MRSA, no antibiotic; lane 6, MRSA plus solithromycin; lane 7, H. influenzae, no antibiotic; lane 8, H. influenzae plus solithromycin. The positions of the 23S and 16S rRNAs are indicated. Enhanced degradation of the rRNAs can be seen as smaller RNA species in the antibiotic-treated samples.

Ribosomal subunit synthesis rates for S. pneumoniae (a), MSSA (b), MRSA (c), and H. influenzae (d). 30S and 50S subunit synthesis rates in the presence or absence of solithromycin at the IC₅₀ are shown. The mean 30S subunit assembly rate with and without antibiotic treatment is shown. The percentage of the total gradient radioactivity in the subunit at each time point is indicated. Standard errors are indicated (n = 2).