PMC

Spectra of three different laser lines taken on a grating spectrograph coupled to a CCD detector.

Avidin-coated beads labeled with different biotinylated SERS tags were analyze by spectral flow cytometry. A) Spectra of individual beads B) average particle spectra for each SERS tag. C) Contribution of each SERS tag as estimated using CLS spectral unmixing.

Conventional detection uses dichroic mirrors and bandpass filters to select colors of light for detection on PMTs. Spectral detection uses gratings or prisms to disperse light across a detector array.

A) Spectral flow cytometry data from multifluorphore beads (UltraRainbow, Spherotech). Intensity histograms generated by applying virtual bandpass filters: B) 530/30 C) 585/40, D) 610 LP.

Biotinylated beads labeled with different streptavidin QDots were analyze by spectral flow cytometry. A) Spectra of individual beads B) average particle spectra for each QDot. C) Contribution of each QDot as estimated using band pass filters. D) Contribution of each QDot as estimated using CLS spectral unmixing.

A) Spectral flow cytometry data of PE calibration beads (PE Quantibrite, BD Biosciences). B) Histogram of Integrated Emission intensity for the calibration bead set. C) plot of calibration bead MESF value vs Integrated Intensity and the number of photons detected, as calculated from the detector response.