(A) Structure of the PEP1 locus and the position of exon 1a and exon 1b specific primers. (B) Sequence comparisons of FLC and tandem duplicated copies 1a and 1b in accession Paj (grey to blue boxes in ). Vista plot using Calc. window 25, Min cons width 25 and Cons identity 70%. (C) Alignment using part of the 416 bp sequence (yellow box) specific for exon 1a from different FLC homologues. Intr1aR primer was designed in a consensus sequence. (D) PCR test using PEP1a specific primers (Ex1F and Intr1aR) in different accessions. Template used is Dor (1), Tot (2), Wca (3), Cza (4), Mug (5) Paj (6) and water control (7). (E) Alignment using part of the intron sequence downstream of PEP1b. Intr1bR primer was designed in a duplicated region (pink box in , ) conserved in other FLC homologues. (F) PCR test using PEP1b specific primers (Ex1F and Intr1bR) in different accessions. Template used is Dor (1), Tot (2), Wca (3), Cza (4), Mug (5) Paj (6) and water control (7). (G) PEP1 structure of the accessions Paj, Dor, Cza and Tot obtained by sequencing the PEP1 locus.