PMC

Receiver operating characteristic (ROC) curves of differentially expressed miRNAs between TB infected patients and healthy controls. ROC curves of miR-210 (A), miR-26a (B), miR-432 (C) and miR-134 (D) yielded an AUC value less than 0.7.

Receiver operating characteristic (ROC) curves of differentially expressed miRNAs between TB infected patients and healthy controls. ROC curves of miR-361-5p (A), miR-889 (B) and miR-576-3p (C) showed a moderate distinguishing efficiency. The combination of miR-361-5p, miR-889 and miR-576-3p showed a slightly higher AUC value of 0.863 (D).

Ten serum miRNA levels in TB patients and healthy controls were selected for verification using real-time qRT-PCR in individual TB patients(N = 30)and healthy controls(N = 65). Serum levels of miR-361-5p, miR-889, miR-576-3p, miR-210, miR-26a, miR-432 and miR-134 were significantly higher in TB patients compared with those in the control group (**, P < 0.01, * P < 0.05) while no significant differences were detected in the expression of miR-423-5p, miR-335 and miR-144*. Expression levels of the miRNAs are normalized to miR-16 (Log₂ relative level).