PMC

LacCer-BODIPY-FL metabolism in seven individual granule neurons is revealed by capillary electrophoresis. Representative electropherograms are shown at (A) full scale and (B) expanded scale. Several unidentified compounds marked with a question mark are also observed. (Reprinted (adapted) with permission from []. Copyright 2012 American Chemical Society)

Live single-cell mass spectrometry was used to examine metabolite content in a variety of cells, including leaf cells from Pelargonium zonale. A representative mass spectrum of sample collected from an individual cell using a nanoelectrospray tip (tip is encircled). Adapted with permission from []. Copyright 2012 American Chemical Society.

Analyses of vacuolar and cytoplasmic phosphate metabolites of Chara australis based on hierarchical cluster analysis shows that they are distinct. Some dependence on the 24 h light/dark cycle is detected. Three major data clusters represent cytoplasm-type metabolites (clusters 1 and 2) and vacuole-type metabolites (cluster 3). Reprinted from an open access source [].

Sequential investigation of samples with several analytical technologies yields increased chemical information content and metabolome coverage. The LDI-MS/SIMS/CRM spatial correlation strategy is illustrated using tall perennial grass Miscanthus x giganteus cross sections. The LDI-MS grid (center top) is color-coded, corresponding to the intensity of m/z = 45 ions, obtained by laser desorption/ionization excitation spots on 100 μm centers. The yellow circle highlights the spot where high-resolution imaging was performed by both negative (m/z = 25, C²H^-, top left) and positive (m/z = 43, C³H⁷⁺, bottom left) ion SIMS, as well as CRM, characterized by the cellulose band, 345–390 cm^-1 (top right), and the lignin band, 1550–1650 cm^-1 (bottom right). (Bottom center) Composite CRM image combining information from both cellulose (green) and lignin (yellow) bands. Reprinted with permission from []. Copyright 2010 American Chemical Society.