Venn diagram and OPLS-DA of protein spot expression data. The significantly differentially expressed protein spots have been calculated with OPLS-DA where ABS (loadings)—ABS (jack-knife confidence interval) if positive indicates significance. The model has been applied on 302 protein spots of dilated versus nondilated samples (44 patients with and without TAA) in TAV and BAV separately. The bar plot of loadings and jack-knife confidence intervals contributing to the first PC for significant spots common for TAV and BAV models, red bars (A), statistically significant only in TAV, blue bars, and BAV, green bars (B) and (C) respectively. D, Venn diagram based on ABS (loading)—ABS (jack-knife confidence interval) significant protein spots of dilated versus nondilated samples in TAV and BAV patients separately (derived from Supplemental Table 2), color coded according to (A)–(C). E, Combined model scatter plot where loadings from TAV model of dilated versus nondilated aorta samples is plotted against loadings from BAV model of dilated versus nondilated aorta samples, color coded according to (A)–(C). Proteins that are significantly differentially expressed only in TAV and BAV are marked in blue and green respectively, whereas proteins that show statistically significant differential expression in both TAV and BAV are marked in red. 109 protein spots chosen for identification by MALDI-TOF are marked with numbers in the combined model scatter plot. The black diagonal is aimed for the interpretation purposes.