PMC

Expression of Col I, Col III, Col V, tenomodulin, and matrix metalloproteinase-2 (MMP-2) by human rotator cuff tendon fibroblasts cells. * and # indicate significant difference between groups at a given time point and within a group at consecutive time points, respectively, at p<0.05 (n=4). Color images available online at www.liebertpub.com/tea

Effect of diameter on scaffold mechanical properties. (A) Nano- and microfiber scaffolds (scanning electron microscopy, 0.8 kV, 2000×). (B) Fiber diameter (n=3) increased with solution viscosity (n=4). (C) Effect of fiber diameter on scaffold tensile properties (n=6). “#” indicates significant difference within a group at consecutive values of fiber diameter and “*” indicates significant difference as compared to 320 nm at p<0.05. Red circles indicate points of measurements. PLGA, poly(d,l-lactide-co-glycolide); UTS, ultimate tensile strength. Color images available online at www.liebertpub.com/tea

Effect of scaffold fiber diameter on cell biosynthesis. (A) Cell viability (live/dead, n=3). (B) Aspect ratio of cells over time (n=3). (C) Cell proliferation (n=6). (D) Collagen production (n=6), and (E) Glycosaminoglycan (GAG) production (n=6). * and # indicate significant difference between groups at a given time point and within a group at consecutive time points, respectively, at p<0.05. Color images available online at www.liebertpub.com/tea

Fiber diameter guided fibroblast morphology and attachment. (A) Top panel: vector maps of cells at day 28; bottom panel: mean angle distribution of fibers and cells at day 28. (B) Values of orientation parameters at day 28. #,* and ^ indicate significant difference with respect to monolayer, 320 and 680 nm, respectively, at p<0.05 (n=3). Mean angle: −90°≤θ≤90°, 0°=horizontal orientation; angular deviation: 0°=aligned, 40.5°=random; mean vector length: 0=random, 1=aligned. Color images available online at www.liebertpub.com/tea