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1.
Figure 3

Figure 3. Profiling of the microbial structure in colonic mucus layers by T-RFLP.. From: Lactobacillus reuteri Maintains a Functional Mucosal Barrier during DSS Treatment Despite Mucus Layer Dysfunction.

The columns show representative profiles from the loose (L) and firm (F) colonic mucus layers and treatment groups (control, L. reuteri, L. reuteri+DSS and DSS).

Johan Dicksved, et al. PLoS One. 2012;7(9):e46399.

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2.
Figure 6

Figure 6. Consensus T-RFLP profiles from different sampling sites in the DSS treated animals.. From: Lactobacillus reuteri Maintains a Functional Mucosal Barrier during DSS Treatment Despite Mucus Layer Dysfunction.

TRFs colored in a red color scale were found in loose (L), firm (F) mucus and the mesenteric lymph nodes (MLN), TRFs colored in a green color scale were found in loose mucus and MLN, TRFs colored in a blue scale were unique for the MLN and TRFs colored in grey were only found in mucus.

Johan Dicksved, et al. PLoS One. 2012;7(9):e46399.

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3.
Figure 1

Figure 1. Disease activity index in animals treated with DSS alone or Lactobacillus reuteri+DSS.. From: Lactobacillus reuteri Maintains a Functional Mucosal Barrier during DSS Treatment Despite Mucus Layer Dysfunction.

In the first group, DSS were given in the drinking water for 9 days. In the second, 109 L. reuteri/day were given by gavage for 16 days and DSS were given for the last 9 days of the treatment. *p<0.05 DSS vs. L. reuteri+DSS.

Johan Dicksved, et al. PLoS One. 2012;7(9):e46399.

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4.
Figure 2

Figure 2. Numbers of total anaerobic bacteria and lactobacilli determined by cultivation.. From: Lactobacillus reuteri Maintains a Functional Mucosal Barrier during DSS Treatment Despite Mucus Layer Dysfunction.

For each treatment group (controls, DSS, L. reuteri, L. reuteri+DSS), data are shown for both loosely and firmly adherent colonic mucus. Different letters (a–c) above the bars indicate significant differences in bacterial counts between groups or mucus layers (p<0.05). # Lactobacilli were only detected in two of the three samples.

Johan Dicksved, et al. PLoS One. 2012;7(9):e46399.

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5.
Figure 5

Figure 5. L. reuteri reduces DSS associated translocation.. From: Lactobacillus reuteri Maintains a Functional Mucosal Barrier during DSS Treatment Despite Mucus Layer Dysfunction.

A) Culture data (log cfu g−1 mesenteric lymph node) from mesenteric lymph nodes from the different treatment groups. B) The identity of the dominant culturable bacteria that translocated. Different letters (a–b) above the bars indicate significant differences in bacterial counts between groups (p<0.05). ANOVA followed by Tukey's post hoc test was used for the statistical analysis.

Johan Dicksved, et al. PLoS One. 2012;7(9):e46399.

CitationFull text

6.
Figure 4

Figure 4. Clustering of samples according to its microbial structure.. From: Lactobacillus reuteri Maintains a Functional Mucosal Barrier during DSS Treatment Despite Mucus Layer Dysfunction.

The heatmap shows the relative abundances of TRFs that differed significantly between clusters. The base pair (bp) sizes are indicated in the figure. Each column represents the abundance profile for a sample and each row the abundance profile for a TRF. The abundance interval is shown in a blue color scale and significance levels in a green color scale. †Indicates TRFs that were identified in false discovery rate analysis. Samples were sorted according to clustering using Bray Curtis metrics. Branch color represents: red, DSS; blue, L. reuteri+DSS; green, control; and yellow, L. reuteri. T-RFLP data of loosely adherent mucus (L) and firmly adherent mucus (F) showing one cluster of control and L. reuteri firm mucus samples (right cluster), one cluster of control and L. reuteri loose mucus samples (middle cluster) and one cluster of DSS-treated animals including a mix of both loosely and firmly mucus (left cluster).

Johan Dicksved, et al. PLoS One. 2012;7(9):e46399.

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