Frequency of CS-TCR CD8+ T cells (with specificity for the dominant CD8+ T cell epitope from P. yoelii circumsporozoite protein, PyCSP280–288) within the draining lymph nodes (DLN) and spleen (SPL) seven days after adoptive transfer into congenic hosts and vaccination with a single s.c. injection of lipid core peptides (LCP), or pooled peptides (P1, P2, P3, P4; see ) and CpG-Alum, or i.m. injection with DNA vaccine encoding PyCSP (D7) (A); or seven days after the final of three similar vaccinations given at 3 week intervals (W7) (B). (mean and SEM) with mice tested individually are shown (A, SPL and DLN n = 6 mice pooled data from two repeat experiments; B, SPL n = 5 mice from a representative experiment) except for B, DLN which were pooled from five mice (mean shown). Statistical comparisons are made to a control group that received adoptively transferred cells but no vaccination (No Vacc Ctrl) or as indicated on the graph, using one-way ANOVA with Bonferroni's post-test.