PMC

Polymer immunoblotting and immunocytochemistry of HaCaT keratinocytes grown for 14 days under control (+) or restricted (−) niacin (Nam) and exposed to photodamage. Cells treated with singlet oxygen stress (¹O₂) or solar simulated light (SSL) were analyzed by (A) Western blots using a PAR antibody and (B) immunostaining using a PAR antibody (green) and nuclear counterstaining with DAPI (blue).

SIRT gene expression at 5 hours after singlet oxygen stress treatment was measured using qPCR. (A) NHEK (mean ± SEM, n = 2 independent experiments with triplicate samples). (B) HaCaT cells (mean ± SEM, n = 3 independent experiments with triplicate samples). Gene expression levels shown are expressed as fold-change relative to untreated cells, where all samples were normalized to GAPDH gene expression. *p<0.05 and a fold change >1.5 or <0.67 (dashed lines).

NHEK grown on a collagen-coated membrane were raised to the air-liquid interface (air-lift) to induce differentiation. (A) SIRT gene expression was measured by qPCR as a function of time. Expression levels shown are calculated as fold change relative to expression levels in submerged cultures (before airlift) after normalization to 18 S rRNA expression. Mean ± SEM, n = 4. *p<0.05 and a fold change >1.5 or <0.67 (dashed lines). (B) Hematoxylin and eosin (H&E) and filaggrin immunohistochemical staining of epidermal reconstructs reveals differentiation progress after airlift.

SIRT gene expression profiles in HaCaT keratinocytes were measured using qPCR. (A) Niacin-restricted SIRT gene message levels shown are expressed as fold-change relative to niacin replete HaCaTs. (B) Time course of SIRT1 and SIRT4 mRNA expression after SSL treatment. (C) Time course of SIRT gene expression after singlet oxygen stress. Expression levels shown for B and C are calculated as fold-change relative to untreated niacin-restricted HaCaTs. All samples were normalized to GAPDH gene expression. Mean ± SEM, n = 3 independent experiments with triplicate samples. *p<0.05 and a fold change >1.5 or <0.67 (dashed lines).

DNA damage in HaCaT keratinocytes grown in the presence (control) or absence (restricted) of added niacin for 7 or 14 days was analyzed by alkaline comet assays. Dots represent the tail moment of single cells; mean tail moment is indicated by the lines shown for: (A) untreated, (B) SSL treated, and (C) singlet oxygen stress treated cells. ns, non significant difference; *, denotes significant differences, by unpaired Student’s t test, p<0.001.

Time course of SIRT1 and SIRT4 gene expression after SSL treatment was measured using qPCR. (A) NHEK cells (mean ± SEM, n = 2 independent experiments, each ran in triplicate). (B) HaCaT cells (mean ± SEM, n = 3 independent experiments, each ran in triplicate). Gene expression levels shown are expressed as fold-change relative to untreated cells, where all samples were normalized to GAPDH gene expression. *p<0.05 and a fold change >1.5 or <0.67 (dashed lines). (C) SIRT1 and (D) SIRT4 protein expression in NHEK cells was detected by Western blotting using the antibody described in . To normalize for protein loading, blots were probed with an antibody against α-tubulin. (E) Percentage change in SIRT1 and (F) SIRT4 protein expression level relative to untreated NHEK after normalization to α-tubulin.