PMC

(a) Flow chart of analysis performed by PhenoRipper. (b) PhenoBrowser interface. Upper left: 3-D MDS plot of profiles for images of 3T3-L1 cells on different days of differentiation to adipocytes (days indicated by color). Right column: two selected images from day 15 (top) and 9 (bottom) (blue/green/yellow/red: DNA/lipid droplets/AdipoQ/PPARγ). Lower left: superblock features (lower left) that best distinguish the two selected images. (c) 2D MDS plot of PhenoRipper profiles for images of the 1820 “hits” from a genome-wide siRNA screen performed by Fuchs et al. Colors represent the six tightest phenotypic groups defined by Fuchs et al.; Class: 1-metaphase, 2-high-actin ratio, 3-lamellapodia + high-actin ratio, 4-proliferating cells, 5-small cells, 6-big cells, gray dots – other/unclassified. A.U.: arbitrary units.