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1.
Figure 4

Figure 4. Western blot validation.. From: Changes in Global Gene Expression Associated with 3D Structure of Tumors: An Ex Vivo Matrix-Free Mesothelioma Spheroid Model.

Total protein (50 µg) prepared from NCI-H226 spheroids (3D) and monolayer (2D) cells were loaded to SDS-PAGE followed by Western blot analysis.

Heungnam Kim, et al. PLoS One. 2012;7(6):e39556.
2.
Figure 1

Figure 1. The ex vivo tumor spheroid model.. From: Changes in Global Gene Expression Associated with 3D Structure of Tumors: An Ex Vivo Matrix-Free Mesothelioma Spheroid Model.

Microscopic images of monolayers and spheroids of human mesotheliomoa cell line NCI-H226. 2D: monolayers; 3D: spheroids. Scale: 400 μm.

Heungnam Kim, et al. PLoS One. 2012;7(6):e39556.
4.
Figure 2

Figure 2. Hierarchical clustering of microarray data.. From: Changes in Global Gene Expression Associated with 3D Structure of Tumors: An Ex Vivo Matrix-Free Mesothelioma Spheroid Model.

Differentially expressed 142 probe sets with a five or greater fold change in expression as well as a student's t-test output of p<0.05 were visualized by Genespring program. Red indicates higher expression of the gene in NCI-H226 spheroid cells compared with monolayer cells (up-regulated), and green indicates lower expression of the gene compared with the NCI-H226 monolayer cells (down-regulation). Each row represents the expression level for the transcripts tested for an individual sample. Each column shows the expression levels for a single transcript tested for different samples. The entire list of 142 probes is presented in Table S1. 2D: monolayers; 3D: spheroids.

Heungnam Kim, et al. PLoS One. 2012;7(6):e39556.

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