PMC

Entamoeba moshkovskii was expelled within 2 weeks in CBA/J Mice. CBA/J (open circles) and C57BL/6 (solid circles) mice were intracecally inoculated with 1 × 10⁶ trophozoites of Entamoeba histolytica (A) or Entamoeba moshkovskii (B). Time course of each Entamoeba spp. infection was then monitored by detection of the parasites in stool by culture and polymerase chain reaction (PCR).

Entamoeba moshkovskii induced intestinal symptoms and weight loss in CBA/J mice. CBA/J mice were intracecally inoculated with 1 × 10⁶ trophozoites of E. moshkovskii. After infection, diarrhea, colitis, and weight loss were monitored. Normal (A), loose (B), and bloody feces (C) were observed as was indicated in the results. Amoebae were observed in the lumen of the ceca in successfully infected mice (D). Macroscopic and histopathological observations of ceca in mice exhibited bloody diarrhea were shown in panels E and F. Changes in body weight were monitored in successfully infected 15 mice per group (G), in which CBA/J mice were intracecally inoculated with 1 × 10⁶ trophozoites of Entamoeba histolytica (solid squares), E. moshkovskii (solid circles), or medium alone (open diamonds). The study was repeated 3 times with similar results. *P < 1.0 × 10⁻⁶, **P < 1.0 × 10⁻⁵ and ***P < 1.0 × 10⁻⁴ compared with sham-operated mice (Mann–Whitney U test).