PMC

Effect of Poly (I:C) treatment on CPE. BEAS-2B cells were treated with 4 μg/ml of Poly (I:C) (+) or mock-treated (−) for 1 h, and then infected with CHIKV at MOI 0.01, 1, and 5. At 24, 48, and 72 h p.i., CPE was observed under a microscope.

Effect of Poly (I:C) treatment on CHIKV growth. BEAS-2B cells were treated with 4 μg/ml of Poly (I:C) (□) or mock-treated (■) for 1 h, and then infected with CHIKV at MOI 0.01, 1, and 5. At 24, 48, and 72 h p.i., the virus titer in the supernatant was measured by a plaque assay. *P < 0.01 by Student’s unpaired t-test.

Expression of anti-viral genes after Poly (I:C) treatment. BEAS-2B cells were treated or not treated with Poly (I:C), and a total RNA was extracted from the cells at 0, 2, 4, 8, 16, and 24 h p.i. The IFN-β, IFN-α, MxA, and OAS genes were amplified by RT-PCR, and the products were analyzed by agarose gel electrophoresis.

Induction of IFN-β and expression of TLR3 in BEAS-2B cells treated with Poly (I:C) or infected with CHIKV. (A) BEAS-2B cells were incubated in the presence of 4 μg/ml of Poly (I:C), and IFN-β secreted in the medium was measured by an ELISA. The samples were collected at 0, 2, 4, 8, 16, and 24 p.i. (B) BEAS-2B cells were infected with CHIKV at MOI 0.8. IFN-β in the medium was measured by an ELISA at 0, 2, 4, 8, 16, and 24 h p.i.. * P < 0.05: ** P < 0.01 relative to the 0 h time point. (C) Expression of TLR3 in BEAS-2B cells was detected by RT-PCR. The cells were incubated with 4 μg/ml of Poly (I:C) or infected with CHIKV at MOI 0.8. Total RNA was extracted from the cells at 24 h p.i., and TLR3 mRNA was amplified by RT-PCR. The products were analyzed by agarose gel electrophoresis. A representative result of the experiment performed in triplicate is shown.