PMC

Ckit receptor (red) staining is seen in the cerebellum (left) and hippocampus (right) in sections labeled with GFAP (green, cerebellum) or DCX (green, hippocampus). There were no differences detected between W^sh/+ (top) and W^sh/W^sh (bottom) mice. Low magnification scale bar=100 μm, high magnification inset scale bar=20μm

(A) Representative photomicrographs of DCX staining in the dentate gyrus of W^sh/+ and W^sh/W^sh mice are shown. The small white box shows location of high magnification inset; Scale bars, 100 μm for hippocampal photomicrograph, 12 μm for inset. (B) Histogram shows the area of DCX staining in the dentate gyrus. **, p<0.01.

(A) The number of BrdU+ cells and (B) the area of DCX staining are shown in response to saline and SSRI treatment. Different letters above the histogram bars indicate statistically significant differences, p<0.05.

(A) Representative photomicrographs show BrdU staining in the dentate gyrus (bregma -1.8). (B) The number of BrdU+ cells is shown across the rostral-caudal extent of the dentate gyrus. (C) Histogram shows sum of BrdU+ cells in the dentate gyrus. (D) Representative photomicrographs of BrdU staining in the SVZ are shown. (E) Histogram shows the number of BrdU+ cells in the SVZ. *, p<0.05. scale bars, 100 μm

(A) Histogram shows % BrdU+ cells surviving in the dentate gyrus for 28 days. (B) The % surviving BrdU+ cells that were also NeuN+ is shown. (C) Representative photomicrographs of BrdU staining in the olfactory bulb of W^sh/+ and W^sh/W^sh mice are shown. Scale bar, 300 μm. (D) Histogram shows the number of BrdU+ cells in olfactory bulb. *, p<0.05

(A) Mast cells are stained with toluidine blue (left) and avidin (right). Arrowheads indicate representative double labeled mast cells. (B) Photomicrographs of toluidine blue stained mast cells located within the hippocampal parenchyma (i, iv) or meninges (ii, iii, v, vi) near the hippocampus. Scale bars,100 μm all upper panels; 5 μm for A bottom panels and B iv, v; and 60 μm for B vi.

The contribution of mast cells to serotonin content in the rostral (blue) and caudal (red) hippocampus is shown following stimulation with (A) HBSS vehicle or (B) C48/80. There were no significant differences between genotypes in either rostral or caudal hippocampus following HBSS vehicle application or in rostral hippocampal slices stimulated with C48/80. However, in brain slices from W^sh/+ but not W^sh/W^sh mice, there were increases in serotonin in the caudal hippocampus following stimulation with C48/80. *, p<0.05.

(A) Sagittal view of the location of mast cells in PN1 and adult mice are shown. Each dot represents one mast cell (blue) in relation to the hippocampus (red). Mast cells are located in the parenchyma of the hippocampus as well as the leptomeninges adjacent to the hippocampus. (B) Coronal view of adult brain depicts the intracranial location of mast cells from bregma-2.30 to -3.40mm with each dot representing one mast cell. (C) The total number of intracranial mast cells (x±sem) and (D) the number of mast cells within or near the hippocampal formation is shown throughout development (E) The % of the total population that these mast cells comprise (within and near hippocampus/ total brain mast cells × 100) is shown.

(A) The number of errors in the radial arm maze is shown for training and reversal trials. (B) The percentage of trials in which mice performed a serial search is shown. (C) Escape latency in the Morris water maze is shown over hidden and visible platform trials. (D) Representative swim paths of W^sh/+ and W^sh/W^sh mice during the probe trial are shown. Dashed lines demarcate quadrants and the grey box indicates the location of the platform during training. (E) Percent of time spent in each target and nontarget quadrant of the pool during the probe trial is shown for W^sh/+ and W^sh/W^sh mice. Dashed line indicates chance performance (25%). *, p<0.05; **, p<0.01.