PMC

Heat map showing overall expression patterns of differentially regulated genes from up-regulated libraries. Hierarchical clustering of significantly MJ up-regulated genes was performed using average linkage and Euclidean distance as a measurement of similarity. Only results from probes showing a statistically significant change in expression for at least one time point (P < 0.05) are shown.

Semi-quantitative RT-PCR analysis of 12 selected up-regulated unigenes. Semi-quantitative RT-PCR was carried out using RNA from the Taxus cuspidata P93AF cell line. RNA samples were taken at 6h, 18h and 5 day after MJ elicitation, and from mock elicited cells at the same time points. Lane 1: Mock elicited for 18h, Lane 2-4: elicited with 100 μM MJ for 6h, 18h or 5 day, respectively. Gapdh and Actin1 were used as normalization controls.

Expression pattern of clones from up-regulated libraries. Macroarrays spotted with randomly selected clones (probes) from all six SSH libraries were hybridized with labelled RNA targets prepared from mock elicited and elicited cells at three time points: 6h, 18h, and 5 day. Expression profile plots for the set of probes corresponding to each up-regulated library are shown: a, 6h library probes b, 18h library probes c, 5 day library probes. Expression is given as the fold-change in gene expression in the elicited culture over the un-elicited culture. Only results from probes showing a statistically significant change in expression (P < 0.05) are shown.