PMC

ASFV titer during acute infection in swine. Virus yields at different times post-infection (X axis) are expressed as log₁₀ HAD₅₀/ml (Y axis). Each point represents the mean of viral titer. Error bars indicate the standard errors.

Immature white blood cells during acute ASFV infection. A - lymphoblast, B - eosinophilic metamyelocyte, C - monoblast, D - metamyelocyteBlood slides were stained by Giemsa modified solution. Cells were examined under the light microscope at 1250 × magnification. Scale bar is 10 μm.

Peripheral white blood cells of uninfected pigs. A - eosinophil, B - basophil, C - medium lymphocyte, D - small and large lymphocytes, E - monocyte, F - segmented neutrophil, G, H - band neutrophils. Blood slides were stained by Giemsa modified solution. Cells were examined under the light microscope at 1250 × magnification. Scale bar is 10 μm.

Pathological and non-pathological white blood cells during acute ASFV infection. A, B, C - different forms of reactive lymphocytes, D, F, G - reactive lymphoblasts with vacuoles, E, H - reactive monoblasts with vacuoles, I - plasmocyte, J, K, L, M - atypical lymphocytes with altered nuclear shape, N - atypical lymphocyte, O - hypersegmented neutrophil, P, Q, R - hemagglutination or hemadsorbtion. Blood slides were stained by Giemsa modified solution. Cells were examined under the light microscope at 1250 × magnification. Scale bar is 10 μm.

Distribution of lymphoblasts by ploidy during acute ASFV infection. A - ploidy of lymphoblasts at 3 dpi, B - at 4 dpi, C - at 5 dpi, D - at 6 dpi, E - at 7 dpi, F - the nucleus of lymphoblast stained by the method of Feulgen for ploidy analysis. X axis is the ploidy of cells. Y axis is the percent of distribution of cells. Data are presented from 3 dpi because no changes in ploidy (they were diploid as in control) were seen at 1-3 dpi. Cells were examined at 1250 × magnification. Scale bar is 10 μm.

Distribution of atypical lymphocytes by ploidy during acute ASFV infection. A - ploidy of atypical lymphocytes at 3 dpi, B - at 4 dpi, C - at 5 dpi, D - at 6 dpi, E - at 7 dpi, F - the nucleus of lymphoblast stained by the method of Feulgen for ploidy analysisX axis is the ploidy of cells. Y axis is the percent of distribution of cells. Data are presented from 3 dpi because no changes in ploidy (they were diploid as in control) were seen at 1-3 dpi. Cells were examined at 1250 × magnification. Scale bar is 10 μm.

Distribution of monoblasts by ploidy during acute ASFV infection. A - ploidy of monoblasts at 3 dpi, B - at 4 dpi, C - at 5 dpi, D - at 6 dpi, E - the nucleus of monoblast stained by the method of Feulgen for ploidy analysis. X axis is the ploidy of cells. Y axis is the percent of distribution of cells. Data are presented from 3 dpi because no changes in ploidy (they were diploid as in control) were seen at 1-3 dpi. A few monoblasts, with hypodiploid DNA content, were detected at 7 dpi, and therefore data for 7 dpi are not presented. Cells were examined at 1250 × magnification. Scale bar is 10 μm.