PMC

Synthesis of the AIE monomer (AIEM) and its copolymers.

Polymer concentration dependent fluorescence for CMC determination of P1 (A) and P4 (B) in aqueous solutions.

Cytotoxicity of polymers P1 and P4 to U87MG (A) and CP-A (B) cells. Cellular internalization time is 24 hours at 37 °C.

A) Time dependent flow cytometry of P4 by U87MG cells at 37 °C. B) Time dependent flow cytometry of P4 by CP-A cells at 37 °C. C) Time dependent flow cytometry of P4 by CP-A cells at 4 °C.

Confocal fluorescence images of P4 for CP-A (A, B, C) and U87MG (D, E, F). A and D are fluorescence images from the polymer. B and E are bright field images. C is the overlay of A and B. F is the overlay of D and E.

A) Typical DLS of P1 and P4 in 2% DMSO aqueous solutions and the AFM image of P4 (inserted, 0.75 μm × 0.75 μm). B) A possible schematic drawing of the micelles formed from the polymers using a “flower-micelle model”.

A) Absorbance and emission spectra of a typical polymer P3 in DMSO and 2% DMSO aqueous solution. B) Emission intensity change of P3 in the mixture of DMSO and water. The spectra were excited at 405 nm. C) Quantum yields of polymers P1 to P5 in DMSO and water mixtures.