PMC

Whisker plots of left versus right maximum paw strike force across the 4 groups of capsulotomized controls, untreated, PBS-treated, and human synoviocyte lubricin (HSL)-treated ACL transected rat joints at post-operative day 70. (*p<0.01; **p<0.001)

Representative rat gait with paw strikes identified over 2 gait cycles. The central maximum pressure within each strike box and its frame number is illustrated. Note that left and right forelimbs (LF and RF) superimpose on left and right hindlimbs (LH, RH) during quadruped gait and are differentiated by frame number.

Whisker plots of urinary CTX-II (uCTX-II) levels, normalized to creatinine, from rats with ACLT right hindlimbs randomized to treatment with either HSL or PBS at post-operative day 7. Control animals underwent a sham capsulotomy. Urine was collected for 24hrs from all animals on post-operative days 35 and 70. All uCTXII assays were performed in duplicate. (** p<0.001; * p < 0.01)

Safranin O stained and immunoprobed tibial plateau cartilage from a) capsulotomized, b) untreated, c) PBS-treated, d) human synoviocyte lubricin (HSL)-treated ACLT rat joints. Monoclonal antibodies 3B3(−) and 2B6 probed for chondroitin 6 sulfate (C6S) and chondroitin 4 sulfate(C4S) respectively. Scale bars are 20 μm.

Immunostaining of articular cartilage for lubricin probed with mAb 9G3 and in situ hybridization for lubricin mRNA from representative rats at day 70 post-op from a) capsulotomized b) untreated c) PBS-treated d) human synoviocyte lubricin (HSL)-treated ACLT rat joints. Joints which are untreated or PBS-treated display caspase 3 activity and are TUNEL positive. Scale bars are 20 μm.